Research Interest: Physical Chemistry and Applied Spectroscopy
Specific Research Area: Applications of fluorescence spectroscopy, imaging, probing and single molecule studies towards nanoscopic investigation and bio analysis
Current Research Theme:
How
much could it be crucial to obtain the right information in the molecular scale,
it is equally important to device new tools and methodology for getting the
right information. Exploring new phenomenon in chemistry through advancement of
modern technology was never as easy as it looks today. Applying advanced
spectroscopic tools such as
fluorescence
probing methodology and
single molecule detection techniques
we would like to characterize complicated chemical species and explore
unexplored chemical phenomenon at nanoscopic and microscopic level while
estimating molecular properties like
interactions, distances, dynamics, diffusion rates and paths, aggregations,
orientations, conformational changes,
in solution and at surfaces.
Our research will lead towards single
molecule manipulation for
nanoscopic investigations, bio analysis
and applications in nanobiotechnology.
Subsequently, our study will focus to go beyond present understanding of
chemo-mechanical energy coupling i.e.
how chemical energy is converted to
mechanical force and vice versa and we
will learn more details on Mechano-chemistry.
This investigation might trigger
chemists to design new molecules that can directly convert energy stored in a
chemical bond to mechanical force without choosing any alternative energy path
and providing 100 % efficiency.
Past Research Projects:
(i) Single Molecule Mechano-chemistry, e.g. "Biophysical Study of Rotary motor
like F1-ATPase":- Tools: Single Molcule Imaging, Photon Counting Set up,
Magnetic Tweezers, Protein Expression, Purification & Characterization.
(ii) Single Molecule Instruemntation and Techniques Development:- Tools: Single
Molecule (SM) Wide-field Imaging, SM Confocal Imaging, SM Spectroscopy and SM
Defocused Imaging; Materials: Quantum dots, Organic dyes, Dendrimer, Proteins,
GFPs
(iii) Optimisation Fluorescence Correlation Spectroscopic Measurement:- Tools:
Confocal microscopy, Laser Spectroscopy; Matrials: Organic dyes,
(iv) Time-resolved Life-time Decay, Anisotropy Decay & Spectroscopy:-
Investigation: Structure, Property, and Mechanistic Study of Azo Dyes by Phenols
& Alkylated Benezenes, New Fluorescent Time-resolved Anisotropy Probe for
Micelle, lipid membrane, Proteins
(v) Development of Fiber Optic Fluorescence Sensor & Optical Sensinng:-
Materials: PAHs, Nitarted PAHs, benzo[k]fluoranthene
(vi) Development of Synchronour Fluorescence Scan Spectroscopic Methods:-
Investigation: Multi-component PAHs mixture analysis by developing
multivariate techniques,
spectroscopic evaluation of petroleum products at higher concentration,
development of Total Synchronous Fluorescence Scan Spectroscopic Methods for
Petroleum Products Analysis, Novel method development for estimation of
Adulteration in Petrol & Diesel
(vii) Development of Excitation Emission Matrix Fluorescence Methods:-
Investigation: Spectroscopic evaluation of petroleum products at higher
concentration, development of Total relative quantum yield measurement for
Petroleum Products at higher concentration, Novel method development for
estimation of Adulteration in Petrol & Diesel, Motor oils
(vii) Fluorescence Probing and Spectroscopy:- Materials: Cyanine dyes, Micelle